Knockout/Knockdown Validation

At GeneTex, we understand that researchers need high-quality antibodies to achieve accurate and reproducible experimental results. We recognize the importance of stringent validation of our reagents through the use of various analytic strategies to ensure persistent quality (see GeneTex's QC method to lot-to-lot variability) and confirm antibody specificity. Based on the guidelines described by the International Working Group on Antibody Validation (IWGAV), GeneTex has been employing multiple approaches to certify antibody performance in our quality assurance process:



    Knockout/ Knockdown Knockout/ Knockdown
Complete or significant reduction of signal following genome editing or RNA interference of target protein.
    Comparative antibodies Comparable antibodies
Correlation between two antibodies against the same target with different epitopes across several samples, preferentially with different expression levels of the target protein.
Mass spec analysis of target protein peptide sequences from samples immunoprecipitated by antibody from lysates.
    Biological and orthogonal characteristics
Change of antibody signal in accordance with defined biological characteristics of target protein following specific sample preparation parameters, including drug treatments, hypoxia conditions, or subcellular fractionation. Also, analysis of signals from various cell lines that either do or do not express the target in the context of protein or mRNA levels.
    Protein Overexpression Protein Overexpression
To confirm the endogenous signals detected by the antibody are the target protein, transfected cells with cDNA encoding the target protein are used as a positive control and independently validated by western blot.



To facilitate your search, antibodies that passed at least one of these validation methods are identified by the certification icons.



1. Nat Methods. 2016 Oct;13(10):823-7.