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GRK2 (phospho Ser670) antibody [HL1035]

Recombinant antibody Orthogonal Overexpression
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in IHC (Paraffin sections) (IHC-P). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

Cat. No. GTX635875

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1035

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GRK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser670 of human GRK2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909912

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor kinase 2 , ADRBK1 , BARK1 , BETA-ARK1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in IHC (Paraffin sections) (IHC-P). GTX635875

GTX635875 IHC-P Image

GRK2 (phospho Ser670) antibody [HL1035] detects GRK2 (phospho Ser670) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
GRK2 (phospho Ser670) stained by GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Various tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations