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GRK2 (phospho Ser670) antibody [HL1035]

Recombinant antibody Orthogonal Overexpression
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in IHC (Paraffin sections) (IHC-P). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875
Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

Cat. No. GTX635875

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1035

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GRK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser670 of human GRK2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909912

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

G protein-coupled receptor kinase 2 , ADRBK1 , BARK1 , BETA-ARK1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in IHC (Paraffin sections) (IHC-P). GTX635875

GTX635875 IHC-P Image

GRK2 (phospho Ser670) antibody [HL1035] detects GRK2 (phospho Ser670) protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
GRK2 (phospho Ser670) stained by GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 (phospho Ser670) antibody [HL1035] used in Western Blot (WB). GTX635875

GTX635875 WB Image

Various tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (GTX635875) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations