ATM antibody [2C1]
|Applications||ELISA, FACS, ICC/IF, IHC-P, IP, WB, IHC-R, ChIP assay|
|Application Note||Recommended Starting Dilutions:|
For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa.
For IHC-P: Use at 5 µg/mL. Antigen retrieval in Citrate buffer is recommended.
For IP: Use at a concentration of 1-10 µg/ml.
For ICC/IF: Please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For FACS:Use at an dependent assay.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||370 kDa (note)
|Positive Controls||HeLa , HeLa nuclear extract , NT2D1 , PC-3 , SK-N-SH|
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G affinity purified|
|Purification Note||From mouse ascitic fluid|
|Full Name||ataxia telangiectasia mutated|
|Product Description||Mouse monoclonal antibody [2C1] to ATM|
|Background||The ATM antibody, clone 2C1, recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.|
|Synonyms||ATD, 114480, ATA, AT1, ATC, ATE, DKFZp781A0353, 472, ATM, TEL1, ATDC, Q13315, MGC74674|
|Immunogen||Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.|
|Species Reactivity||Human, Mouse, Monkey, Rat|