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10X ImmunoStain wash buffer

Cat. No. GTX73344

Reference 1

Application

 IHC
Package
1 liter ($179)
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PRODUCT

Summary

10X ImmunoStain wash buffer (pH 7.5)

APPLICATION

Application Note

No pH adjustment or additives are required when using this formulation. Simply dilute the concentrated 10-fold buffer with distilled or deionized water. A total of 10 liters of working rinsing solution can be prepared with 1 liter of the concentrated reagent. This buffer is also the reagent of choice for rinsing tissue sections that are subjected to immunocytochemical staining using conventional horizontal processing techniques.

PROPERTIES

Storage

Store at room temperature.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Optimal immunostaining not only depends on the specificity of the primary antibody and other immunoreagents but also depends on obtaining a good signal to noise ratio. Binding of an antibody to its epitopes involves van der Waals forces, electrostatic forces and hydrophobic forces. Certain antibodies have tendency to bind loosely and nonspecifically to unrelated epitopes, which can create undesired background staining. In order to remove these nonspecifically bound antibodies, a thorough washing is required after each immunostaining step. Immuno Wash Buffer is specifically designed to remove such loosely bound antibodies effectively and efficiently and to provide a cleaner background staining.

REFERENCE

Application Reference

Elkabets M et al. Methods Mol Biol 2018; 1806 : 155-176 Methods to Study the Role of Progranulin in the Tumor Microenvironment.
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Package List Price ($)
$ 179