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Isotype Controls

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding.

These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

 

Human Immunoglobulins

Human Immunoglobulins

Rabbit Immunoglobulins

Rabbit Immunoglobulins

Mouse Immunoglobulins

Mouse Immunoglobulins

 
Rat Immunoglobulins

Rat Immunoglobulins

Chicken Immunoglobulins

Chicken Immunoglobulins

Goat Immunoglobulins

Goat Immunoglobulins

 
Sheep Immunoglobulins

Sheep Immunoglobulins

Hamster Immunoglobulins

Hamster Immunoglobulins

   
Literature
Cardiovascular Research

Pamphlet - Cardiovascular Research

Download the latest version of GeneTex's cardiovascular research pamphlet.
Articles

Five Pillars of Antibody Validation

GeneTex employs various analytic validation strategies to ensure both consistent quality and specificity.
Articles

Knockout/Knockdown Validation

GeneTex is conducting knockout/knockdown-based validation as a fundamental component of its antibody quality assurance process.
Protocols

Protocol - ELISA (sandwich)

Download GeneTex's ELISA (sandwich) Protocol.
Protocols

Protocol - ELISA (indirect)

Download GeneTex's ELISA (indirect) Protocol.
Literature
Cardiovascular Research

Pamphlet - Cardiovascular Research

Download the latest version of GeneTex's cardiovascular research pamphlet.
Protocols

Protocol - ELISA (sandwich)

Download GeneTex's ELISA (sandwich) Protocol.
Protocols

Protocol - ELISA (indirect)

Download GeneTex's ELISA (indirect) Protocol.