Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] (GTX109090) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] (GTX109090) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 epsilon(GTX109090) antibody at 1:200 dilution.

14-3-3 epsilon antibody [N1C3] immunoprecipitates YWHAE protein in IP experiments.
IP samples: HeLa whole cell extract
A. 40 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of YWHAE protein by 4 μg 14-3-3 epsilon antibody [N1C3] (GTX109090)
12 % SDS-PAGE
The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] (GTX109090) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon(GTX109090) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min