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14-3-3 theta antibody [3B9]

Anti-14-3-3 theta antibody [3B9] used in Western Blot (WB). GTX10439

Cat No. GTX10439

Host Mouse
Clonality Monoclonal
Clone Name 3B9
Isotype IgG1
Application WB, IP
Reactivity Human, Mouse, Rat, Bovine
25 μg ($289)

Application Note

For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:5,000. Detects a band of approximately 31 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

28 kDa. ( Note )

Positive Control

3T3, PC12, and Jurkat






20 mM sodium phosphate, 150 mM sodium chloride, 50% Glycerol,pH 7.5


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.


1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species



Recombinant full length protein.


Protein G purified




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Theta , 14-3-3 , 1C5 , Hs1 , Ywhaq


At least seven isoforms comprise the highly conserved 14-3-3 family of homo and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. These multifunctional proteins bind and modulate the function of a wide array of cellular proteins. They exist mainly as dimers with a monomeric molecular mass of approximately 30 kDa and have an acidic isoelectric point of 4.5-5. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with bad, prevents apoptosis. 14-3-3 plays a dual role in Raf-1 activation. It helps to keep Raf-1 in an inactive state by binding to phophoserine-259 and the cysteine-rich domain (DRD). Interactions of 14-3-3 at the phosphoserine-621 may be required for Raf1 activation and may function as an essential cofactor for Raf1 kinase activity. Other proteins that have been shown to bind 14-3-3s include members of the protein kinase C family, Cbl, IRS1, middle-T antigen, KSR, IGF1 receptor, etc. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases. The theta or tau isoform was initially isolated from T cells. It's structure has been resolved. It is found in T cells, brain and testes. Although the zeta isoform is present at high levels, beta, gamma, tau and eta isoforms are also expressed in the rat brain. However, the tau isoform is the only isoform found in the white matter.


Anti-14-3-3 theta antibody [3B9] used in Western Blot (WB). GTX10439

GTX10439 WB Image

At right is a typical western blot (10% SDS-PAGE) of 3T3 (lane 1), PC12 (lane 2), MDBK (lane 3), Jurkat (lane 4) and normal human fibroblasts (lane 5).

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Package List Price ($)
$ 289