Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of 4E-BP1 protein from HCT116 whole cell extracts using 5 μg of 4E-BP1 antibody [N1C3] (GTX109162) or 4E-BP1 antibody [N1C3-2] (GTX116315).
Western blot analysis was performed using 4E-BP1 antibody [N1C3] (GTX109162).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody (GTX109162) diluted at a dilution of 1:2000.

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using EIF4EBP1(GTX109162) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min