5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

Cat. No. GTX629765
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Cat. No.  GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765
GTX629765 MeDIP Image

GTX629765 5-HydroxymethylCytidine antibody [GT13612] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

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Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot (Dot). GTX629765
GTX629765 Dot Image

GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

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Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

5 / 5
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot (Dot). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    GT13612
  • Isotype
    IgG2b
  • Applications
    ICC/IF IHC-P IHC-Fr Dot EM MeDIP
  • Reactivity
    Species independent
Summary
5-hmC antibody recognizes 5-Hydroxymethylcytosine (5-hmC), which is referred to as a “sixth base” of mammalian genomic DNA. 5-hmC is thought to be an epigenetic regulatory modification in its own right, while also being the product of the first TET protein-catalyzed oxidation of 5-methylcytosine in the DNA demethylation pathway.
See all 5-Hydroxymethylcytosine / 5-hmC products

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