GeneTex
United States (US)

A-549 (human lung carcinoma) cell line slides

Cat No. GTX25527

Application ICC/IF
Species Human
PRODUCT

Product Description

A-549 cell line slides (human: lung; carcinoma)

Tissue type

Tumor
APPLICATION

Application Note

A549 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested.
PROPERTIES

Storage

Stable for up to 6 months at 4˚C under desiccate conditions.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

A549 (lung; carcinoma)

Human A549 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The A549 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 A549 cells. A follow-up heat dehydration process was done to ensure the attachment of A549 cells and the stability of cellular proteins.

Package List Price ($)
$ 199