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A1CF antibody, N-term

Anti-A1CF antibody, N-term used in Western Blot (WB). GTX47219

Cat No. GTX47219

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
50 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human A1CF

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

APOBEC1 complementation factor , ACF , ACF64 , ACF65 , APOBEC1CF , ASP

Cellular Localization

Nucleus,Endoplasmic reticulum,Cytoplasm

Background

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Database

DATA IMAGES

Anti-A1CF antibody, N-term used in Western Blot (WB). GTX47219

GTX47219 WB Image

WB analysis of HepG2 cells using GTX47219 A1CF antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379