A-431 (epidermis; epidermoid carcinoma)
Human A-431 cells were cultured in DMEM medium and harvested at the log phase of growth. The A-431 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 A-431 cells. A follow-up heat dehydration process was done to ensure the attachment of A-431 cells and the stability of cellular proteins.