ACE2 antibody [SN0754]

Name: ACE2 antibody [SN0754]
Brand: GeneTex
SKU: GTX01160
Price: 399.00 USD

Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160

Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160

Anti-ACE2 antibody [SN0754] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01160

Cat. No. GTX01160

Reference 12

Host	Rabbit
Clonality	Monoclonal
Clone Name	SN0754
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, African green monkey
Package	100 μl ($399)

See all ACE2 products

View product citations for antibody GTX01160 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500~1:5000
ICC/IF	1:100-1:500
IHC-P	1:50-1:200

Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Observed MW

130 kDa. The observed M.W. is based on the following publication. PMID: 24417403

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide within Human ACE2 aa 200-230.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACE2 , ACEH

Cellular Localization

Secreted,Cell membrane,Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Gene ID: 59272 ACE2
UniProt: Q9BYF1 ACE2

Research Area

DATA IMAGES

GTX01160 WB Image

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human intestine.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX01160 WB Image

Untreated (–) and treated (+) Vero E6 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX01160 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24417403

GTX01160 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX01160 ICC/IF Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min