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ACE2 antibody [SN0754]

Recombinant antibody KO/KD Orthogonal
Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160
Anti-ACE2 antibody [SN0754] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01160
Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160
Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160
Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160
Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160
Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160
Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160
Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160
Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160
Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160

Cat No. GTX01160

Reference 9
1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 SN0754

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, African green monkey
Package
100 μl ($379)
See all ACE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500~1:5000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Observed MW

130 kDa. The observed M.W. is based on the following publication. PMID: 24417403

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide within Human ACE2 aa 200-230.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

angiotensin I converting enzyme 2 , ACE2 , ACEH

Cellular Localization

Secreted,Cell membrane,Cytoplasm

Background

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160

GTX01160 WB Image

KO/KD

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ACE2 antibody [SN0754] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01160

GTX01160 ICC/IF Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160

GTX01160 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24417403

Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160

GTX01160 WB Image

Orthogonal

Untreated (–) and treated (+) Vero E6 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human intestine.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160

GTX01160 IHC-P Image

ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160

GTX01160 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [SN0754] used in IHC (Paraffin sections) (IHC-P). GTX01160

GTX01160 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01160 ACE2 antibody [SN0754].

REFERENCE

Application Reference

Sherman EJ et al. PLoS Pathog 2022; 18 (3) : e1010377 Identification of cell type specific ACE2 modifiers by CRISPR screening.
Application : WB
Reactivity : Human
Miao-Hsi Hsieh et al. Front Immunol 2021; 12 : 641360 Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles.
Application : WB, ICC/IF
Emily J Sherman et al. Sci Rep 2021; 11 (1) : 15900 ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomes.
Application : WB
Reactivity : Human
Hsin-I Wang et al. Int J Mol Sci 2021; 22 (16) Small Structural Proteins E and M Render the SARS-CoV-2 Pseudovirus More Infectious and Reveal the Phenotype of Natural Viral Variants.
Application : WB, ICC/IF
Reactivity : Human
Chiu-Yang Lee et al. Int J Mol Sci 2021; 22 (18) Tumor Necrosis Factor-Alpha Exacerbates Viral Entry in SARS-CoV2-Infected iPSC-Derived Cardiomyocytes.
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Submit a Reference

REVIEW

ACE2 antibody [SN0754] Cat No. GTX01160

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-ACE2 antibody [SN0754] used in Western Blot (WB). GTX01160
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in J Cell Mol Med. PMID: 34821008
Submit a Review
Application Reference
Sherman EJ et al. PLoS Pathog 2022; 18 (3):e1010377 Identification of cell type specific ACE2 modifiers by CRISPR screening.
Application : WB
Reactivity : Human
Armin Bayati et al. J Biol Chem 2021;:100306 SARS-CoV-2 infects cells after viral entry via clathrin-mediated endocytosis.
Application : WB, ICC/IF
Miao-Hsi Hsieh et al. Front Immunol 2021; 12:641360 Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles.
Application : WB, ICC/IF
Emily J Sherman et al. Sci Rep 2021; 11 (1):15900 ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomes.
Application : WB
Reactivity : Human
Hsin-I Wang et al. Int J Mol Sci 2021; 22 (16) Small Structural Proteins E and M Render the SARS-CoV-2 Pseudovirus More Infectious and Reveal the Phenotype of Natural Viral Variants.
Application : WB, ICC/IF
Reactivity : Human
Chiu-Yang Lee et al. Int J Mol Sci 2021; 22 (18) Tumor Necrosis Factor-Alpha Exacerbates Viral Entry in SARS-CoV2-Infected iPSC-Derived Cardiomyocytes.
Generali M et al. J Cell Mol Med 2021; Heterogeneous expression of ACE2 and TMPRRS2 in mesenchymal stromal cells.
Application : WB
Ahmad Mulyadi Lai HI et al. Int J Mol Sci 2021; 22 (3) Expression of Endogenous Angiotensin-Converting Enzyme 2 in Human Induced Pluripotent Stem Cell-Derived Retinal Organoids.
Application : ICC/IF
Reactivity : Human
Qiao Y et al. Proc Natl Acad Sci U S A 2020; (Epub) Targeting transcriptional regulation of SARS-CoV-2 entry factors ACE2 and TMPRSS2.
Application : IHC-P
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379