APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500~1:5000 |
1:100-1:500 |
1:50-1:200 |
Not tested in other applications.
Calculated MW
Observed MW
130 kDa. The observed M.W. is based on the following publication. PMID: 24417403
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide within Human ACE2 aa 200-230.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
angiotensin I converting enzyme 2 , ACE2 , ACEH
Cellular Localization
Secreted,Cell membrane,Cytoplasm
Background
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX01160 WB Image
Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX01160 ICC/IF Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunofluorescent analysis.Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.Green: ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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GTX01160 WB Image
Untreated (–) and treated (+) Vero E6 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01160 IHC-P Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX01160 IHC-P Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human intestine.ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX01160 IHC-P Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX01160 IHC-P Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX01160 IHC-P Image
ACE2 antibody [SN0754] detects ACE2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.ACE2 stained by ACE2 antibody [SN0754] (GTX01160) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX01160 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01160 WB Image
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [SN0754] (GTX01160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 24417403
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GTX01160 IHC-P Image
IHC-P analysis of human kidney tissue section using GTX01160 ACE2 antibody [SN0754].
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REFERENCE
There are currently no references for ACE2 antibody [SN0754] (GTX01160). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ACE2 antibody [SN0754] (GTX01160). Be the first to share your experience with this product.