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ACTH antibody [2F6]

Anti-ACTH antibody [2F6] used in IHC (Paraffin sections) (IHC-P). GTX34389

Cat No. GTX34389

Host

Mouse

Clonality

Monoclonal

Clone Name

2F6

Isotype

IgG1

Application

ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Specificity/Sensitivity

This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH).

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to aa1-24 of human ACTH

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ACTH , CLIP , LPH , MSH , NPP , POC , POMC , proopiomelanocortin , Corticotropin

Cellular Localization

Secreted

Background

This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-ACTH antibody [2F6] used in IHC (Paraffin sections) (IHC-P). GTX34389

GTX34389 IHC-P Image

IHC-P analysis of human pituitary gland tissue using GTX34389 ACTH antibody [2F6].

REFERENCE

There are currently no references for ACTH antibody [2F6] (GTX34389). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ACTH antibody [2F6] (GTX34389). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf