GeneTex
United States (US)

ACVRL1 antibody

Cat No. GTX10598

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2  μg/ml
ELISA 0.5-1.0  μg/mL
Not tested in other applications.

Calculated MW

56 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 5% trehalose

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

recombinant human ALK-1 extracellular domain expressed in mouse NSO cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Activin A Receptor Like Type 1,Acvrlk1,Alk-1,Alk1,Hht,Hht2,Orw2,Skr3,Tsr-I,Acvrl1

Background

The TGF-beta superfamily of ligands exerts its biological activity by binding to heteromeric receptor complexes two types (I and II) of the serine/threonine kinases. Type II receptors are constitutively active kinases, which phosphorylate type I receptors after ligand binding. In turn, the activated type I kinases phosphorylate downstream signaling molecules including the various smads. Seven type I receptors, also termed activin receptor-like kinase (ALK), have been isolated from mammals. The physiological ligand for ALK1 is currently unknown. It is suggested that TGF-beta 1 and TGF-beta 3 can activate chimeric ALK1. ALK1, like the other type I receptors, contains a cysteine rich domain with conserved cysteine spacing in the extracellular region and a glycine and serine rich domain preceding the kinase domain. The reduced human ALK1 monomer has a calculated molecular mass of approximately 37.3 kDa. Due to glycoslyation, the recombinant human protein migrates as a 50-55 kDa protein in SDS-PAGE under reducing conditions. Human and mouse ALK1 share approximately 71% amino acid sequence identity in their extracellular domains. ALK1 is highly expressed in endothelial cells and other vascular tissues. Mutations in ALK1 are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting an important role for ALK1 in the control of blood vessel development and repair.

Database

Research Area