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AChE antibody [HL1102]

Recombinant antibody Comparison Orthogonal
Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298
Anti-AChE antibody [HL1102] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636298
Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298
Anti-AChE antibody [HL1102] used in IHC (Paraffin sections) (IHC-P). GTX636298
Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298
Anti-AChE antibody [HL1102] used in IHC (Paraffin sections) (IHC-P). GTX636298
Anti-AChE antibody [HL1102] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636298

Cat. No. GTX636298

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1102

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat
Package
100 μl ($399),
25 μl ($169)
See all AChE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Bovine, Guinea pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human AChE. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909963

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acetylcholinesterase (Cartwright blood group) , ACEE , ARACHE , N-ACHE , YT

Cellular Localization

Cell junction, synapse,Secreted,Cell membrane

Background

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298

GTX636298 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-AChE antibody [HL1102] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636298

GTX636298 ICC/IF Image

AChE antibody [HL1102] detects AChE protein at dendrite by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298

GTX636298 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AChE antibody [HL1102] used in IHC (Paraffin sections) (IHC-P). GTX636298

GTX636298 IHC-P Image

AChE antibody [HL1102] detects AChE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298

GTX636298 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AChE antibody [HL1102] used in IHC (Paraffin sections) (IHC-P). GTX636298

GTX636298 IHC-P Image

AChE antibody [HL1102] detects AChE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AChE antibody [HL1102] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636298

GTX636298 ICC/IF Image

AChE antibody [HL1102] detects AChE protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations