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AChE antibody

Anti-AChE antibody used in IHC (Frozen sections) (IHC-Fr). GTX101648
Anti-AChE antibody used in Western Blot (WB). GTX101648
Anti-AChE antibody used in IHC (Paraffin sections) (IHC-P). GTX101648
Anti-AChE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101648
Anti-AChE antibody used in IHC (Paraffin sections) (IHC-P). GTX101648
Anti-AChE antibody used in Western Blot (WB). GTX101648
Anti-AChE antibody used in IHC (Paraffin sections) (IHC-P). GTX101648
Anti-AChE antibody used in Western Blot (WB). GTX101648

Cat No. GTX101648

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Raji , mouse brain , rat brain

Predict Reactivity

Bovine, Cat, Guinea pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human AChE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

acetylcholinesterase (Cartwright blood group) , ACEE , ARACHE , N-ACHE , YT

Cellular Localization

Cell junction , synapse , Secreted , Cell membrane; Peripheral membrane protein , Isoform T: Nucleus , Isoform H: Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Cell junction , synapse , Secreted , Cell membrane , Isoform T: Nucleus , Isof

Background

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AChE antibody used in IHC (Frozen sections) (IHC-Fr). GTX101648

GTX101648 IHC-Fr Image

AChE antibody detects AChE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: AChE protein stained by AChE antibody (GTX101648) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AChE antibody used in Western Blot (WB). GTX101648

GTX101648 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody (GTX101648) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AChE antibody used in IHC (Paraffin sections) (IHC-P). GTX101648

GTX101648 IHC-P Image

AChE antibody detects AChE protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
AChE antibody (GTX101648) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AChE antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101648

GTX101648 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AChE(GTX101648) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-AChE antibody used in IHC (Paraffin sections) (IHC-P). GTX101648

GTX101648 IHC-P Image

AChE antibody detects AChE protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
AChE antibody (GTX101648) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AChE antibody used in Western Blot (WB). GTX101648

GTX101648 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody (GTX101648) diluted at 1:500.

Anti-AChE antibody used in IHC (Paraffin sections) (IHC-P). GTX101648

GTX101648 IHC-P Image

AChE antibody detects AChE protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
AChE antibody (GTX101648) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AChE antibody used in Western Blot (WB). GTX101648

GTX101648 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX101648 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169
Currently in production