GeneTex
United States (US)

AChE antibody

GTX101648_42564_20160921_IHC-Fr_19011506_649.jpg
GTX101648_39911_20160825_WB_M_R_19011506_979.jpg
GTX101648_39911_IHC_R_19011506_199.jpg
GTX101648_39911_IFA_19011506_980.jpg
GTX101648_39911_20160323_IHC-P_R_19011506_279.jpg
GTX101648_42564_20160825_WB_R_brain_19011506_150.jpg
GTX101648_39911_IHC_M_19011506_141.jpg
GTX101648_WB_19011506_339.jpg

Cat No. GTX101648

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Raji , mouse brain , rat brain

Predict Reactivity

Bovine, Cat, Guinea pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human AChE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARACHE antibody, N-ACHE antibody, YT antibody, ACHE antibody, Yt blood group antibody, acetylcholinesterase antibody, apoptosis-related acetylcholinesterase antibody

Cellular Localization

Cell junction , synapse , Secreted , Cell membrane; Peripheral membrane protein , Isoform T: Nucleus , Isoform H: Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Cell junction , synapse , Secreted , Cell membrane , Isoform T: Nucleus , Isof

Background

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101648_42564_20160921_IHC-Fr_19011506_649.jpg

GTX101648 IHC-Fr Image

AChE antibody detects AChE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: AChE protein stained by AChE antibody (GTX101648) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101648_39911_20160825_WB_M_R_19011506_979.jpg

GTX101648 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody (GTX101648) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101648_39911_IHC_R_19011506_199.jpg

GTX101648 IHC-P Image

AChE antibody detects AChE protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
AChE antibody (GTX101648) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101648_39911_IFA_19011506_980.jpg

GTX101648 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AChE(GTX101648) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX101648_39911_20160323_IHC-P_R_19011506_279.jpg

GTX101648 IHC-P Image

AChE antibody detects AChE protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
AChE antibody (GTX101648) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101648_42564_20160825_WB_R_brain_19011506_150.jpg

GTX101648 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody (GTX101648) diluted at 1:500.

GTX101648_39911_IHC_M_19011506_141.jpg

GTX101648 IHC-P Image

AChE antibody detects AChE protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
AChE antibody (GTX101648) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101648_WB_19011506_339.jpg

GTX101648 WB Image

Sample (30 µg of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX101648 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169