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ADAM17 antibody [HL1572]

Recombinant antibody
Anti-ADAM17 antibody [HL1572] used in Western Blot (WB). GTX637047
Anti-ADAM17 antibody [HL1572] used in IHC (Paraffin sections) (IHC-P). GTX637047
Anti-ADAM17 antibody [HL1572] used in Western Blot (WB). GTX637047

Cat No. GTX637047

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1572

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAM17. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADAM metallopeptidase domain 17 , ADAM18 , CD156B , CSVP , NISBD , NISBD1 , TACE

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-ADAM17 antibody [HL1572] used in Western Blot (WB). GTX637047

GTX637047 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [HL1572] (GTX637047) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ADAM17 antibody [HL1572] used in IHC (Paraffin sections) (IHC-P). GTX637047

GTX637047 IHC-P Image

ADAM17 antibody [HL1572] detects ADAM17 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ADAM17 stained by ADAM17 antibody [HL1572] (GTX637047) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ADAM17 antibody [HL1572] used in Western Blot (WB). GTX637047

GTX637047 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [HL1572] (GTX637047) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for ADAM17 antibody [HL1572] (GTX637047). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations