Preservative: 0.02% Sodium Azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Synthetic peptide unique to PAC4 protein was used to generate antibodies in rabbit. All peptides were amidated before conjugation.
Immunogen affinity purified
The affinity-purified antibodies are isolated on antigen-sepharose affinity column and supplied IgG in antibody stabilization buffer.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Integral membrane protein
The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors (GPCRs). Using the high inter-species homology of mammalian AC isoforms, nine Adenylyl cyclase (AC) isoforms, encoded by separate genes, have been identified until today. Human adenylate cyclase genes comprise of 11 to 26 exons, which are distributed over 16 to 430kb. The expression profile of these 9 AC isoforms in a panel of 16 human tissues and in human embryonic kidney (HEK) cells have been demonstrated earlier. Stimulation of adenylate cyclases produce cAMP form ATP in response to the activation of GPCrs by various hormones, neurotransmitters and other regulatory molecules. cAMP, in subsequent steps down the signal transduction pathway, can stimulate cAMP-dependent protein kinase A (cPKA), and several other target molecules. Activation of cPKA can phosphorylate a broad range of substrates that in turn can regulate various metabolic pathways, gene expression, and affect memory functions etc. The stimulation of adenylate cyclases starts with interactions with GPCRs mediated signals initiated by Gs and Gi hetero-trimeric G-proteins. The interaction of GPCR agonist (eg. Interaction of isopreternol to beta2 receptors) catalyses the exchange of of GDP to GTP that is bound to G protein. The GTP binding reduces the affinity of Gs to other GTP binding proteins and Gs-GTP complex stimulate the adenylate cyclase. The cAMP synthesizing enzymes are found in two forms: cytosolic (soluble) and membrane-bound (particulate). In last several years, new members of particulate and soluble adenylate cyclase family have been identified and significant progress is made in understanding of the molecular mechanisms that underlie the regulation of these families of enzymes. Adenylate cyclase Type 4 is located on chromosome type 11.