GeneTex
United States (US)

ADCY9 antibody

ADCY9-antibody_WB_GTX14783-1_18121409_136.jpg

Cat No. GTX14783

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

WB: Use at a dilution of 1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 150 kDa). Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

The antibody does not cross reacts with other adenylate cyclases (AC1 to 8).
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Synthetic peptide unique to PAC9 protein was used to generate antibodies in rabbit. (unfortunately, the amino acid sequence is considered to be commercially sensitive). All peptides were amidated before conjugation.

Purification

Immunogen affinity purified
The antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Integral membrane protein.

Background

The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors (GPCRs). Using the high inter-species homology of mammalian AC isoforms, nine Adenylyl cyclase (AC) isoforms, encoded by separate genes, have been identified until today. Human adenylate cyclase genes comprise of 11 to 26 exons, which are distributed over 16 to 430kb. The expression profile of these 9 AC isoforms in a panel of 16 human tissues and in human embryonic kidney (HEK) cells have been demonstrated earlier. The cAMP synthesizing enzymes are found in two forms: cytosolic (soluble) and membrane-bound (particulate). Stimulation of adenylate cyclases produce cAMP form ATP in response to the activation of GPCRs by various hormones, neurotransmitters and other regulatory molecules. cAMP, in subsequent steps down the signal transduction pathway, can stimulate cAMP-dependent protein kinase A (cPKA), and several other target molecules. Activation of cPKA can phosphorylate a broad range of substrates that regulate various metabolic pathways, gene expression, and affect memory functions etc. The stimulation of adenylate cyclases starts with interactions with GPCRs mediated signals initiated by Gs and Gi heterotrimeric G-proteins. The interaction of GPCR agonist (eg. Interaction of isopreternol to beta2 receptors) catalyses the exchange of GDP to GTP that is bound to G proteins. The GTP binding reduces the affinity of Gs'N to other GTP binding proteins and Gs-GTP complex stimulate the adenylate cyclase. In last several years, new members of particulate and soluble adenylate cyclase family have been identified and significant progress is made in understanding of the molecular mechanisms that underlie the regulation of these families of enzymes.

Research Area

DATA IMAGES
ADCY9-antibody_WB_GTX14783-1_18121409_136.jpg

GTX14783 WB Image

Western blot of Adenylyl cyclase 9 (AC9) antibody with GTX14783. Antibody dilution (1:1000) in DilObuffer. MWM are on the right.

Package List Price ($)
$ 319