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ADFP antibody [ADFP/1493]

Anti-ADFP antibody [ADFP/1493] used in IHC (Paraffin sections) (IHC-P). GTX34397

Cat No. GTX34397

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 ADFP/1493

Isotype

 IgG2b

Application

 IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($339)
See all ADFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1-2μg/ml for 30 minutes at RT
ELISA Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADFP , ADRP , PLIN2 , perilipin 2 , Perilipin 2 , Adipophilin

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-ADFP antibody [ADFP/1493] used in IHC (Paraffin sections) (IHC-P). GTX34397

GTX34397 IHC-P Image

IHC-P analysis of human placenta tissue using GTX34397 ADFP antibody [ADFP/1493].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339