APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml for 30 minutes at RT |
| Assay dependent |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ADFP , ADRP , PLIN2 , perilipin 2 , Perilipin 2 , Adipophilin
Cellular Localization
Membrane
Background
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
![Anti-ADFP antibody [ADFP/1493] used in IHC (Paraffin sections) (IHC-P). GTX34397](/templates/default/js/lightbox/images/loading.gif "Anti-ADFP antibody [ADFP/1493] used in IHC (Paraffin sections) (IHC-P). GTX34397")
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GTX34397 IHC-P Image
IHC-P analysis of human placenta tissue using GTX34397 ADFP antibody [ADFP/1493].
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REFERENCE
There are currently no references for ADFP antibody [ADFP/1493] (GTX34397). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ADFP antibody [ADFP/1493] (GTX34397). Be the first to share your experience with this product.
![Anti-ADFP antibody [ADFP/1493] used in IHC (Paraffin sections) (IHC-P). GTX34397](/upload/website/prouct_img/small/GTX34397/GTX34397_20200115_IHC-P_958_w_23060801_775.webp "Anti-ADFP antibody [ADFP/1493] used in IHC (Paraffin sections) (IHC-P). GTX34397")
![Anti-ADFP antibody [ADFP/1493] used in IHC (Paraffin sections) (IHC-P). GTX34397](/upload/website/prouct_img/normal/GTX34397/GTX34397_20200115_IHC-P_958_w_23060801_775.webp "Anti-ADFP antibody [ADFP/1493] used in IHC (Paraffin sections) (IHC-P). GTX34397")