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AEC Chromogenic kit

Cat No. GTX16204

Application IHC
Package
1 kit ($59)
PRODUCT

Content

AEC Chromogenic kit Package Quantity Volume

Chromogenic reagent A : AEC Concentrated solution

1 3ml
Chromogenic reagent B : Acetate Concentrated Buffer (containing H₂O₂, pH5.6) 1 3ml

 

Product Description

AEC is a widely used chromogen for immunohistochemical staining. AEC produces a red end product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media.
APPLICATION

Application Note

1. After the peroxidase detection, wash with TBS or PBS for 4 times, 5 minutes each.
2. Add one drop of chromogenic reagent A and B respectively into 1 ml of distilled water and mix well. Add it onto the sample and colorate in dark for 10-30 minutes.
3. Wash the slide gently with distilled water to stop the reaction. Counterstain with methyl blue for 3-5 minutes or nonalcoholic hematoxylin (Mayer’s) if necessary.
4. Seal the slide with aqueous mounting medium or glycerol. Observe with microscope.

Note : Counterstain with alcoholic hematoxylin is not recommended.
PROPERTIES

Form

Liquid

Storage

Store at -20ºC for one year.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

AEC is one of the most important chromogenic reagents for peroxidase. The coloration which is showed to be fresh red, and applies to immunohistochemistry and various dot detection. Easy to solution in organic impregnant is its defect, to seal slide with water soluble sealing reagent in case of using AEC is recommend.
REFERENCE
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Package List Price ($)
$ 59