Wild-type (WT) and AKAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKAP1 antibody [N1N2], N-term (GTX115067) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using AKAP1(GTX115067) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX115067 diluted at 1:10000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AKAP1(GTX115067) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min