Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375
Predict Reactivity
Mouse, Rat, Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Rhesus Monkey(>80% identity)
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Akt1/2/3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885774
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
AKT antibody , MGC99656 antibody , PKB antibody , PKB-ALPHA antibody , PRKBA antibody , RAC antibody , RAC-ALPHA antibody , AKT1 antibody , protein kinase B alpha antibody , rac protein kinase alpha antibody , RAC-alpha serine/threonine-protein kinase antibody , proto-oncogene c-Akt antibody , RAC-PK-alpha antibody , PKB alpha antibody , v-akt murine thymoma viral oncogene homolog 1 antibody , PKBB antibody , PKBBETA antibody , PRKBB antibody , RAC-BETA antibody , AKT2 antibody , protein kinase B beta antibody , murine thymoma viral (v-akt) homolog-2 antibody , protein kinase Akt-2 antibody , RAC-PK-beta antibody , PKB beta antibody , rac protein kinase beta antibody , RAC-beta serine/threonine-protein kinase antibody , v-akt murine thymoma viral oncogene homolog 2 antibody , DKFZp434N0250 antibody , PKB-GAMMA antibody , PKBG antibody , PRKBG antibody , RAC-PK-gamma antibody , RAC-gamma antibody , STK-2 antibody , AKT3 antibody , PKB gamma antibody , RAC-gamma serine/threonine-protein kinase antibody , RAC-gamma serine/threonine protein kinase antibody , v-akt murine thymoma viral oncogene homolog 3 (protein kinase B , gamma) antibody
Cellular Localization
Cytoplasm , Nucleus , Cell membrane
Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
Research Area
