GeneTex

  • Country / Location Selection

United States (US)

AKT antibody

Orthogonal
Anti-AKT antibody used in Western Blot (WB). GTX13990

Cat. No. GTX13990

Reference 3

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
50 μg ($489)
See all AKT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immunogen Sequence: GI # 62241011, sequence 2-200 Recombinant serine/threonine protein kinase

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_368804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

AKT (Protein Kinase B, PKB) was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of Akt has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.

Database

Research Area

DATA IMAGES

Anti-AKT antibody used in Western Blot (WB). GTX13990

GTX13990 WB Image

Orthogonal

The data was published in the journal Evid Based Complement Alternat Med in 2012.PMID: 23320038

REFERENCE

Application Reference

Sinanoglu O et al. Urology 2012; 80 (6) : 1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.
Application : WB
Reactivity : Mouse
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1) : 104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Application : WB
Reactivity : Mouse
Hsu HY et al. Evid Based Complement Alternat Med 2012; 2012 : 819632 Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for AKT antibody (GTX13990). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489