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AKT (phospho Ser473) antibody

Orthogonal
GTX128414_41248_20150327_IP_18121410_685.jpg
GTX128414_41766_20180518_WB_R_18121410_638.jpg
GTX128414_41766_20161124_WB_VEGF_18121410_757.jpg
GTX128414_41766_20161124_WB_PMA_18121410_242.jpg
GTX128414_41766_20161124_WB_M_PDGF_18121410_835.jpg
GTX128414_43397_20181123_WB_treatment_CIP_19021719_583.jpg

Cat No. GTX128414

Reference 22
1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
See all AKT1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

PC-12 , Rat2 , MCF-7 , MCF-7(500 nM PMA treatment for 1 hr) , MCF-7(500 nM PMA treatment for 2 hr) , MCF-7(500 nM PMA treatment for 4 hr) , MCF-7(500 nM PMA treatment for 6 hr) , HUVEC , HUVEC (5 ng/ml VEGF treatment for 10 min) , NIH-3T3 , NIH-3T3 (starvation for 16 hr and 5 ng/ml PDGF treatment for 15 min)

Specificity/Sensitivity

This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472.

Predict Reactivity

Zebrafish, Sheep, Cat, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human AKT (phospho Ser473/474/472). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, AKT1 antibody, protein kinase B alpha antibody, rac protein kinase alpha antibody, RAC-alpha serine/threonine-protein kinase antibody, proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, PKB alpha antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, AKT2 antibody, protein kinase B beta antibody, murine thymoma viral (v-akt) homolog-2 antibody, protein kinase Akt-2 antibody, RAC-PK-beta antibody, PKB beta antibody, rac protein kinase beta antibody, RAC-beta serine/threonine-protein kinase antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, DKFZp434N0250 antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AKT3 antibody, PKB gamma antibody, RAC-gamma serine/threonine-protein kinase antibody, RAC-gamma serine/threonine protein kinase antibody, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX128414_41248_20150327_IP_18121410_685.jpg

GTX128414 IP Image

Immunoprecipitation of Akt1/2/3 (phospho Ser473/474/472) protein from 293T whole cell extracts using 5 μg of Akt1/2/3 (phospho Ser473/474/472) antibody (GTX128414).
Western blot analysis was performed using Akt1/2/3 (phospho Ser473/474/472) antibody (GTX128414).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX128414_41766_20180518_WB_R_18121410_638.jpg

GTX128414 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX128414_41766_20161124_WB_VEGF_18121410_757.jpg

GTX128414 WB Image

Orthogonal

Untreated (–) and treated (+) HUVEC whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX128414_41766_20161124_WB_PMA_18121410_242.jpg

GTX128414 WB Image

Orthogonal

MCF-7 cells were untreated or treated with 500 nM PMA for 1, 2, 4 and 6 hrs. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX128414_41766_20161124_WB_M_PDGF_18121410_835.jpg

GTX128414 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX128414_43397_20181123_WB_treatment_CIP_19021719_583.jpg

GTX128414 WB Image

Orthogonal

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
REFERENCE

Application Reference

Lin JT et al. Journal of Functional Foods 2018; 47 : 343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Huang L.C. et al. Disease Markers 2018; 2018 : Article ID 3835783 CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer.
Application : WB
Reactivity : Human
Wu PY et al. Int J Mol Sci 2018; 19 (4) Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells.
Application : WB
Reactivity : Mouse
Wang JP et al. Oncol Lett 2017; 14 (3) : 3503-3509 Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Lin JG et al. Int J Mol Sci 2017; 18 (9) Electroacupuncture Promotes Recovery of Motor Function and Reduces Dopaminergic Neuron Degeneration in Rodent Models of Parkinson's Disease.
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REVIEW

AKT (phospho Ser473) antibody Cat No. GTX128414

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 14-Apr-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1500, 4°C, overnightHr
Submit a Review
Application Reference
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Huang L.C. et al. Disease Markers 2018; 2018:Article ID 3835783 CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer.
Application : WB
Reactivity : Human
Wu PY et al. Int J Mol Sci 2018; 19 (4) Sesamol Inhibited Melanogenesis by Regulating Melanin-Related Signal Transduction in B16F10 Cells.
Application : WB
Reactivity : Mouse
Liu J et al. Oncol Lett 2017; 14 (5):5711-5718 miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
Wang JP et al. Oncol Lett 2017; 14 (3):3503-3509 Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Lin JG et al. Int J Mol Sci 2017; 18 (9) Electroacupuncture Promotes Recovery of Motor Function and Reduces Dopaminergic Neuron Degeneration in Rodent Models of Parkinson's Disease.
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
Application : WB
Reactivity : Human
Tsai CH et al. Front. Pharmacol. 2017;:Epub ahead of print A Standardized Wedelia chinensis Extract Overcomes the Feedback Activation of HER2/3 Signaling upon Androgen-Ablation in Prostate Cancer.
Application : IHC-P
Reactivity : Mouse
Ni YL et al. Apoptosis 2017; (Epub ahead of print) A potent indolylquinoline alleviates growth of human lung cancer cell tumorspheres.
Application : WB
Reactivity : Human
Viedma-Rodriguez R et al. Mol Cell Biochem 2017; (Epub ahead of print) Epsilon-aminocaproic acid prevents high glucose and insulin induced-invasiveness in MDA-MB-231 breast cancer cells, modulating the plasminogen activator system.
Application : WB
Reactivity : Human
Liu J et al. Biotechnol Lett 2017; (Epub ahead of print) MiR-338-3p inhibits TNF-α-induced lipogenesis in human sebocytes.
Application : WB
Reactivity : Human
Kuo YH et al. BMC Complement Altern Med 2017; 17 (1):71 N-(4-methoxyphenyl) caffeamide-induced melanogenesis inhibition mechanisms.
Application : WB
Reactivity : Mouse
Chen PH et al. Neuropharmacology 2016; (Epub ahead of print) The CHAC1-inhibited Notch3 pathway is involved in temozolomide-induced glioma cytotoxicity.
Application : WB
Reactivity : Human
Chen CC et al. PLoS One 2016; 11 (12):e0168092 Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
Application : WB
Reactivity : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Application : WB
Reactivity : Human
Hsieh CH et al. Drug Des Devel Ther 2016; 10:2947-2957 A triazole derivative elicits autophagic clearance of polyglutamine aggregation in neuronal cells.
Application : WB
Reactivity : Human
Chen CC et al. PLoS One 2016; 11 (7):e0158662 The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
Application : WB
Reactivity : Mouse
Albury TM et al. Neoplasia 2015; 17 (2):175-82 Constitutively active Akt1 cooperates with KRas(G12D) to accelerate in vivo pancreatic tumor onset and progression.
Application : WB
Reactivity : Human, Mouse
Chao TT et al. Oncotarget 2015; 6 (4):2164-79 Afatinib induces apoptosis in NSCLC without EGFR mutation through Elk-1-mediated suppression of CIP2A.
Application : IHC-P
Reactivity : Human
Chen CY et al. Oncotarget 2015; 6 (29):26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
Gabriela Vallejo-Flores et al. BioMed Research International 2015; Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation
Application : WB, IP
Reactivity : Human
Sun W et al. Cell Death Dis 2014; 5:e1020 Interleukin-1β promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169