Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX110613 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody (GTX110613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (20 ug of whole cell lysate)
A: HeLa cell lysate
B: HeLa cell transfect with AKT
7.5% SDS PAGE
GTX110613 diluted at 1:10000

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Akt1(GTX110613) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Akt1 antibody immunoprecipitates Akt1 protein in IP experiments.
IP samples: 30 μg whole cell extract of Akt1-transfected 293T cells.
A. 30 μg whole cell extract of Akt1-protein expressing 293T cell
B. Control with 3 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Akt1 protein by 3 μg Akt1 antibody (GTX110613)
10 % SDS-PAGE
The immunoprecipitated Akt1 protein was detected by Akt1 antibody (GTX110613) diluted at 1:5000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]