Sample (20 ug of whole cell lysate)
A: HeLa cell lysate
B: HeLa cell transfect with AKT
7.5% SDS PAGE
GTX111612 diluted at 1:5000

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Akt2(GTX111612) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Akt2 antibody detects Akt2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
Akt2 antibody (GTX111612) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min