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AKT2 antibody

Anti-AKT2 antibody used in Western Blot (WB). GTX111612
Anti-AKT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111612
Anti-AKT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111612

Cat. No. GTX111612

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AKT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HeLa cell transfect with AKT

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Akt2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617856

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AKT serine/threonine kinase 2 , HIHGHH , PKBB , PKBBETA , PRKBB , RAC-BETA

Background

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKT2 antibody used in Western Blot (WB). GTX111612

GTX111612 WB Image

Sample (20 ug of whole cell lysate)
A: HeLa cell lysate
B: HeLa cell transfect with AKT
7.5% SDS PAGE
GTX111612 diluted at 1:5000

Anti-AKT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111612

GTX111612 IHC-P Image

Akt2 antibody detects Akt2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
Akt2 antibody (GTX111612) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AKT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111612

GTX111612 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Akt2(GTX111612) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

Application Reference

Vazquez-Mellado MJ et al. Life Sci 2015; 137 (Epub) : 142-9 erythropoietin protects neuroblastoma cells against * and * by activating erk and akt pathways but has no effect in kidney cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169