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ALDH1A1 antibody [ALDH1A1/1381]

Anti-ALDH1A1 antibody [ALDH1A1/1381] used in Western Blot (WB). GTX34400
Anti-ALDH1A1 antibody [ALDH1A1/1381] used in IHC (Paraffin sections) (IHC-P). GTX34400
Anti-ALDH1A1 antibody [ALDH1A1/1381] used in IHC (Paraffin sections) (IHC-P). GTX34400

Cat No. GTX34400

Host Mouse
Clonality Monoclonal
Clone Name ALDH1A1/1381
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Specificity/Sensitivity

ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ALDH1A1 (aa 315-434) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aldehyde Dehydrogenase 1 Family Member A1 , Aldc , Aldh-E1 , Aldh1 , Aldh11 , Hel-9 , Hel-S-53E , Hel12 , Pumb1 , Raldh1 , Aldh1A1

Background

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES
Anti-ALDH1A1 antibody [ALDH1A1/1381] used in Western Blot (WB). GTX34400

GTX34400 WB Image

Western Blot of K562 and Lung Lysate using ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).

Anti-ALDH1A1 antibody [ALDH1A1/1381] used in IHC (Paraffin sections) (IHC-P). GTX34400

GTX34400 IHC-P Image

Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).

Anti-ALDH1A1 antibody [ALDH1A1/1381] used in IHC (Paraffin sections) (IHC-P). GTX34400

GTX34400 IHC-P Image

Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).

REFERENCE
There are currently no references for ALDH1A1 antibody [ALDH1A1/1381] (GTX34400). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 429