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AMBRA1 antibody

Anti-AMBRA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17003
Anti-AMBRA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17003
Anti-AMBRA1 antibody used in Western Blot (WB). GTX17003
Anti-AMBRA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17003

Cat No. GTX17003

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF Assay dependent
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , 3T3 (NIH) Cell Lysate(GTX27179)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1.The immunogen is located within the last 50 amino acids of Ambra1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887550

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

autophagy and beclin 1 regulator 1 , DCAF3 , WDR94

Database

Research Area

DATA IMAGES

Anti-AMBRA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17003

GTX17003 ICC/IF Image

ICC/IF analysis of K562 cells using GTX17003 AMBRA1 antibody.
Dilution : 20 μg/ml

Anti-AMBRA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17003

GTX17003 IHC-P Image

IHC-P analysis of human brain tissue using GTX17003 AMBRA1 antibody.
Working concentration : 5 μg/ml

Anti-AMBRA1 antibody used in Western Blot (WB). GTX17003

GTX17003 WB Image

WB analysis of 3T3 cell lysate using GTX17003 AMBRA1 antibody.
Working concentration : 1 μg/ml

Anti-AMBRA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX17003

GTX17003 IHC-P Image

IHC-P analysis of human brain tissue using GTX17003 AMBRA1 antibody.
Working concentration : 20 μg/ml

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf