For ELISA: Use at an assay dependent dilution. For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the end user.
Recognizes endogenous human AMPK-alpha only when activated by phosphorylation at Thr172 at 62kD. Detects both alpha-1 and alpha-2 isoforms of the catalytic subunit. It does not detect the regulatory beta or gamma subunits.
10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.113 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide - surrounding Thr172 of human AMPK-alpha (KLH coupled)
Protein A affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Protein Kinase Amp-Activated Catalytic Subunit Alpha 1,Ampk,Ampka1,Prkaa1
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and it plays a key role in regulation of energy homeostasis. The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. AMPK also has a role in apoptosis. AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172. AMPK phosphorylation by AMPKK raises AMPK activity at least 50-fold. Secondly, AMP also inhibits the dephosphorylation and inactivation of AMPK by PP2C.