GeneTex
United States (US)

AMPK alpha 1 (phospho Thr172) antibody

Cat No. GTX30766

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human, Mouse, Rat, Monkey
APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

64 kDa. ( Note )

Specificity/Sensitivity

Recognizes endogenous human AMPK-alpha only when activated by phosphorylation at Thr172 at 62kD. Detects both alpha-1 and alpha-2 isoforms of the catalytic subunit. It does not detect the regulatory beta or gamma subunits.
PROPERTIES

Form

Liquid

Buffer

10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.113 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - surrounding Thr172 of human AMPK-alpha (KLH coupled)

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Kinase Amp-Activated Catalytic Subunit Alpha 1,Ampk,Ampka1,Prkaa1

Background

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and it plays a key role in regulation of energy homeostasis. The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. AMPK also has a role in apoptosis. AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172. AMPK phosphorylation by AMPKK raises AMPK activity at least 50-fold. Secondly, AMP also inhibits the dephosphorylation and inactivation of AMPK by PP2C.

Database

Research Area

Package List Price ($)
$ 319