*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Recognizes endogenous human AMPK-alpha at ~62kD only when activated by phosphorylation at Thr172. Detects both alpha-1 and alpha-2 isoforms of the catalytic subunit. Does not detect the regulatory beta or gamma subunits.
Bovine, Chicken, Pig(>80% identity)
10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
Synthetic phosphopeptide corresponding to residues surrounding Thr172 of human AMPK-alpha.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
AMPK , AMPKa1 , PRKAA1 , protein kinase AMPactivated catalytic subunit alpha 1 , AMPK alpha 1 , protein kinase AMP-activated catalytic subunit alpha 1
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]