GeneTex
  • Country / Location Selection

United States (US)

AMPK alpha 1 (phospho Thr172) antibody

Anti-AMPK alpha 1 (phospho Thr172) antibody used in Western Blot (WB). GTX30766

Cat No. GTX30766

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
50 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Specificity/Sensitivity

Recognizes endogenous human AMPK-alpha at ~62kD only when activated by phosphorylation at Thr172. Detects both alpha-1 and alpha-2 isoforms of the catalytic subunit. Does not detect the regulatory beta or gamma subunits.

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr172 of human AMPK-alpha.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AMPK , AMPKa1 , PRKAA1 , protein kinase AMPactivated catalytic subunit alpha 1 , AMPK alpha 1 , protein kinase AMP-activated catalytic subunit alpha 1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 (phospho Thr172) antibody used in Western Blot (WB). GTX30766

GTX30766 WB Image

WB analysis of untreated or AICAR-treated C2C12 cell extracts using GTX30766 AMPK alpha 1 (phospho Thr172) antibody.

REFERENCE

There are currently no references for AMPK alpha 1 (phospho Thr172) antibody (GTX30766). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for AMPK alpha 1 (phospho Thr172) antibody (GTX30766). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Package List Price ($)
$ 329