AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody

Name: AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody
Brand: GeneTex
SKU: GTX52341
Price: 399.00 USD
Rating: 5 (3 reviews)

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX52341

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341

Cat. No. GTX52341

Reference 11

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Mouse, Rat
Package	100 μg ($399)

See all AMPK alpha 1 products

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW

64 kDa. ( Note )

Product Note

The sequence around AMPK alpha 1 (phospho Thr183) is conserved with the sequence around AMPK alpha 2 (phospho Thr172).AAPK1

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172

Purification

Antigen affinity purification

Conjugation

Unconjugated

RRID

AB_2887793

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , AMPK , AMPKa1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5562 PRKAA1
UniProt: Q13131 PRKAA1

Research Area

DATA IMAGES

GTX52341 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX52341 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 IHC-P Image

IHC-P analysis of rat skin tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 IHC-P Image

IHC-P analysis of human colon tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 ICC/IF Image

ICC/IF analysis of PFA-fixed C2C12 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% saponin
Dilution : 1:400

GTX52341 IHC-P Image

IHC-P analysis of rat lung tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 ICC/IF Image

ICC/IF analysis of PFA-fixed 293 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

GTX52341 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

GTX52341 WB Image

WB analysis of PANC1 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

GTX52341 WB Image

Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

Application Reference

Vadhan A et al. Oncol Lett 2023; 25 (1) : 42 Fumarate hydratase inhibits non-small cell lung cancer metastasis via inactivation of AMPK and upregulation of DAB2.

Application : WB

Reactivity : Human

Hsing-I Tseng et al. Mol Oncol 2022; 16 (11) : 2274-2294 A novel AMPK activator shows therapeutic potential in hepatocellular carcinoma by suppressing HIF1α-mediated aerobic glycolysis.

Application : WB, IHC-P

Reactivity : Human

Ilaria Zuliani et al. Int J Mol Sci 2021; 22 (7) High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures.

Application : WB

Reactivity : Mouse

Lu KY et al. Biochim Biophys Acta Mol Cell Biol Lipids 2021; 1866 (12) : 159033 N-butylidenephthalide ameliorates high-fat diet-induced obesity in mice and promotes browning through adrenergic response/AMPK activation in mouse beige adipocytes.

Application : WB

Reactivity : Mouse

Kang DH et al. Oncol Lett 2021; 21 (2) : 164 Expression of AMP-activated protein kinase/ten-eleven translocation 2 and their clinical relevance in colorectal cancer.

Application : IHC-P

Reactivity : Human

Show More +

Submit a Reference

REVIEW

AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody Cat. No. GTX52341

Rating

( Average 5 based on 3 users reviews)

Application

Western Blot(WB)		( Average 5 based on 2 users reviews)
Immunohistochemistry(IHC)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 24-Jul-2023

Score :

Application Tested : WB

Sample Species : Hu

Figure Note : The data was peer reviewed and published in the 2020 in PLoS Pathog. PMID: 32392268

Date : Anonymous submitted on 06-Sep-2021

Score :

Application Tested : WB

Sample Species : Hu

Sample : Huh7 cells

Amount used : 30 μg

Blocking : 5% BSA in TBST, RT°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

Secondary Antibody : GTX300119, 1:1000, RT°C, 1Hr

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Immunohistochemistry (IHC). GTX52341

Date : Anonymous submitted on 18-Mar-2021

Score :

Application Tested : IHC

Sample Species : Ms

Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 30405100

Submit a Review

Application Reference

Vadhan A et al. Oncol Lett 2023; 25 (1):42 Fumarate hydratase inhibits non-small cell lung cancer metastasis via inactivation of AMPK and upregulation of DAB2.

Application : WB

Reactivity : Human

Hsing-I Tseng et al. Mol Oncol 2022; 16 (11):2274-2294 A novel AMPK activator shows therapeutic potential in hepatocellular carcinoma by suppressing HIF1α-mediated aerobic glycolysis.

Application : WB, IHC-P

Reactivity : Human

Ilaria Zuliani et al. Int J Mol Sci 2021; 22 (7) High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures.

Application : WB

Reactivity : Mouse

Lu KY et al. Biochim Biophys Acta Mol Cell Biol Lipids 2021; 1866 (12):159033 N-butylidenephthalide ameliorates high-fat diet-induced obesity in mice and promotes browning through adrenergic response/AMPK activation in mouse beige adipocytes.

Application : WB

Reactivity : Mouse

Kang DH et al. Oncol Lett 2021; 21 (2):164 Expression of AMP-activated protein kinase/ten-eleven translocation 2 and their clinical relevance in colorectal cancer.

Application : IHC-P

Reactivity : Human

Qiuhua Yang et al. Front Cell Dev Biol 2020; 8:611354 Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders.

Application : IHC-P

Reactivity : Mouse

Zuliani I et al. Neurotherapeutics 2020; (Epub) The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome.

Application : WB

Reactivity : Mouse

Simanjuntak Y et al. PLoS Pathog 2020; 16 (5):e1008521 Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice.

Application : WB

Reactivity : Human

Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281 Metformin triggers the intrinsic apoptotic response in human AGS gastric adenocarcinoma cells by activating AMPK and suppressing mTOR/AKT signaling.

Yang Q et al. Nat Commun 2018; 9 (1):4667 PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis.

Reactivity : Mouse

Yeh YT et al. Nutrients 2017; 9 (10) Chinese Olive (Canarium album L.) Fruit Extract Attenuates Metabolic Dysfunction in Diabetic Rats.

Application : WB

Reactivity : Rat

SDS
Glycerol.pdf
Sodium Azide.pdf

Package	List Price ($)
100 μg	$ 399
Qty

AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody

Cat. No. GTX52341

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX52341 WB Image

GTX52341 IHC-P Image

GTX52341 IHC-P Image

GTX52341 IHC-P Image

GTX52341 IHC-P Image

GTX52341 ICC/IF Image

GTX52341 IHC-P Image

GTX52341 ICC/IF Image

GTX52341 IHC-P Image

GTX52341 IHC-P Image

GTX52341 IHC-P Image

GTX52341 IHC-P Image

GTX52341 WB Image

GTX52341 WB Image

REFERENCE

Application Reference

REVIEW

AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody Cat. No. GTX52341

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.