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AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody

Orthogonal
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX52341
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

Cat. No. GTX52341

Reference 10
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all AMPK alpha 1 products

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW

64 kDa. ( Note )

Product Note

The sequence around AMPK alpha 1 (phospho Thr183) is conserved with the sequence around AMPK alpha 2 (phospho Thr172).AAPK1

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172

Purification

Antigen affinity purification

Conjugation

Unconjugated

RRID

AB_2887793

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase AMP-activated catalytic subunit alpha 1 , AMPK , AMPKa1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX52341

GTX52341 WB Image

Orthogonal

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of human colon tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of rat skin tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX52341

GTX52341 WB Image

WB analysis of PANC1 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341

GTX52341 ICC/IF Image

ICC/IF analysis of PFA-fixed 293 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52341

GTX52341 ICC/IF Image

ICC/IF analysis of PFA-fixed C2C12 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% saponin
Dilution : 1:400

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX52341

GTX52341 WB Image

Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in IHC (Paraffin sections) (IHC-P). GTX52341

GTX52341 IHC-P Image

IHC-P analysis of rat lung tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

REFERENCE

Application Reference

Hsing-I Tseng et al. Mol Oncol 2022; 16 (11) : 2274-2294 A novel AMPK activator shows therapeutic potential in hepatocellular carcinoma by suppressing HIF1α-mediated aerobic glycolysis.
Application : WB, IHC-P
Reactivity : Human
Ilaria Zuliani et al. Int J Mol Sci 2021; 22 (7) High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures.
Application : WB
Reactivity : Mouse
Lu KY et al. Biochim Biophys Acta Mol Cell Biol Lipids 2021; 1866 (12) : 159033 N-butylidenephthalide ameliorates high-fat diet-induced obesity in mice and promotes browning through adrenergic response/AMPK activation in mouse beige adipocytes.
Application : WB
Reactivity : Mouse
Kang DH et al. Oncol Lett 2021; 21 (2) : 164 Expression of AMP-activated protein kinase/ten-eleven translocation 2 and their clinical relevance in colorectal cancer.
Application : IHC-P
Reactivity : Human
Qiuhua Yang et al. Front Cell Dev Biol 2020; 8 : 611354 Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders.
Application : IHC-P
Reactivity : Mouse
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Submit a Reference

REVIEW

AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody Cat. No. GTX52341

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Western Blot (WB). GTX52341
Date : Anonymous submitted on 06-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 30 μg
Blocking : 5% BSA in TBST, RT°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Secondary Antibody : GTX300119, 1:1000, RT°C, 1Hr
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody used in Immunohistochemistry (IHC). GTX52341
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 30405100
Submit a Review
Application Reference
Hsing-I Tseng et al. Mol Oncol 2022; 16 (11):2274-2294 A novel AMPK activator shows therapeutic potential in hepatocellular carcinoma by suppressing HIF1α-mediated aerobic glycolysis.
Application : WB, IHC-P
Reactivity : Human
Ilaria Zuliani et al. Int J Mol Sci 2021; 22 (7) High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures.
Application : WB
Reactivity : Mouse
Lu KY et al. Biochim Biophys Acta Mol Cell Biol Lipids 2021; 1866 (12):159033 N-butylidenephthalide ameliorates high-fat diet-induced obesity in mice and promotes browning through adrenergic response/AMPK activation in mouse beige adipocytes.
Application : WB
Reactivity : Mouse
Kang DH et al. Oncol Lett 2021; 21 (2):164 Expression of AMP-activated protein kinase/ten-eleven translocation 2 and their clinical relevance in colorectal cancer.
Application : IHC-P
Reactivity : Human
Qiuhua Yang et al. Front Cell Dev Biol 2020; 8:611354 Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders.
Application : IHC-P
Reactivity : Mouse
Zuliani I et al. Neurotherapeutics 2020; (Epub) The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome.
Application : WB
Reactivity : Mouse
Simanjuntak Y et al. PLoS Pathog 2020; 16 (5):e1008521 Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice.
Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281 Metformin triggers the intrinsic apoptotic response in human AGS gastric adenocarcinoma cells by activating AMPK and suppressing mTOR/AKT signaling.
Yang Q et al. Nat Commun 2018; 9 (1):4667 PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis.
Reactivity : Mouse
Yeh YT et al. Nutrients 2017; 9 (10) Chinese Olive (Canarium album L.) Fruit Extract Attenuates Metabolic Dysfunction in Diabetic Rats.
Application : WB
Reactivity : Rat
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399