Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

WB analysis of PANC1 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

IHC-P analysis of mouse testis tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

IHC-P analysis of human gastric cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

IHC-P analysis of human colorectal cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

IHC-P analysis of human mammary cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

ICC/IF analysis of PFA-fixed 293 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

IHC-P analysis of rat lung tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

ICC/IF analysis of PFA-fixed C2C12 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% saponin
Dilution : 1:400

IHC-P analysis of human colon tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

IHC-P analysis of rat skin tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

IHC-P analysis of rat spleen tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

IHC-P analysis of mouse kidney tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100

The data was published in the 2020 in PLoS Pathog. PMID: 32392268

The data was published in the journal Nat Commun in 2018. PMID: 30405100