AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 30 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 μg of AMPK alpha 2 antibody (GTX103487) 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody (GTX103487) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 2 antibody (GTX103487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
AMPK alpha 2 antibody (GTX103487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
AMPK alpha 2 antibody (GTX103487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX103487 diluted at 1:1000

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm, nucleus and vesicle by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AMPK alpha 2 stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).