IHC-P analysis of human breast carcinoma tissue using GTX15878 AP2 alpha antibody [3B5].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

ICC/IF analysis of HeLa cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of MCF-7 cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of U251 cells using GTX15878 AP2 alpha antibody [3B5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

WB analysis of whole cell extracts (30 μg lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5) using GTX15878 AP2 alpha antibody [3B5].
Dilution : 1:50-1:200