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APE1 antibody [N1], N-term

Anti-APE1 antibody [N1], N-term used in Western Blot (WB). GTX100050
Anti-APE1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100050
Anti-APE1 antibody [N1], N-term used in Western Blot (WB). GTX100050
Anti-APE1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100050

Cat No. GTX100050

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human APE1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

apurinic/apyrimidinic endodeoxyribonuclease 1,APE,APE1,APEN,APEX,APX,HAP1,REF1

Cellular Localization

Nucleus

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-APE1 antibody [N1], N-term used in Western Blot (WB). GTX100050

GTX100050 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody [N1], N-term (GTX100050) diluted at 1:2000.

Anti-APE1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100050

GTX100050 IHC-P Image

APE1 antibody [N1], N-term detects APEX1 protein at nucleus on Saos2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Saos2 xenograft.
APE1 antibody [N1], N-term (GTX100050) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-APE1 antibody [N1], N-term used in Western Blot (WB). GTX100050

GTX100050 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX100050 diluted at 1:3000

Anti-APE1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100050

GTX100050 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1(GTX100050) antibody at 1:200 dilution.

REFERENCE
There are currently no references for APE1 antibody [N1], N-term (GTX100050). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for APE1 antibody [N1], N-term (GTX100050). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf