Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody [N1], N-term (GTX100050) diluted at 1:2000.

APE1 antibody [N1], N-term detects APEX1 protein at nucleus on Saos2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Saos2 xenograft.
APE1 antibody [N1], N-term (GTX100050) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX100050 diluted at 1:3000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1(GTX100050) antibody at 1:200 dilution.