GeneTex
United States (US)

APE1 antibody [N1], N-term

KO/KD
GTX100050_39855_20160908_WB_shRNA_watermark_19011506_206.jpg
GTX100050_39855_IHC_19011506_357.jpg
GTX100050_WB_19011506_387.jpg
GTX100050_IFA_19011506_694.jpg

Cat No. GTX100050

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.04mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human APE1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, APEX1 antibody, apurinic-apyrimidinic endonuclease 1 antibody, deoxyribonuclease (apurinic or apyrimidinic) antibody, apurinic/apyrimidinic (abasic) endonuclease antibody, redox factor-1 antibody, protein REF-1 antibody, AP endonuclease class I antibody, AP lyase antibody, DNA-(apurinic or apyrimidinic site) lyase antibody, APEX nuclease (multifunctional DNA repair enzyme) 1 antibody

Cellular Localization

Nucleus

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100050_39855_20160908_WB_shRNA_watermark_19011506_206.jpg

GTX100050 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody [N1], N-term (GTX100050) diluted at 1:2000.

GTX100050_39855_IHC_19011506_357.jpg

GTX100050 IHC-P Image

APE1 antibody [N1], N-term detects APEX1 protein at nucleus on Saos2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Saos2 xenograft.
APE1 antibody [N1], N-term (GTX100050) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100050_WB_19011506_387.jpg

GTX100050 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX100050 diluted at 1:3000

GTX100050_IFA_19011506_694.jpg

GTX100050 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1(GTX100050) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169