APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human APP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240465
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4
Cellular Localization
Membrane , Membrane , clathrin-coated pit
Background
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101336 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody [C2C3], C-term (GTX101336) diluted at 1:500.
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GTX101336 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP(GTX101336) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101336 ICC/IF Image
APP antibody [C2C3], C-term detects APP protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APP protein stained by APP antibody [C2C3], C-term (GTX101336) diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX101336 IHC-P Image
APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis. Sample: Paraffin-embedded Rat brain. APP antibody [C2C3], C-term (GTX101336) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101336 WB Image
Sample(30 μg of whole cell lysate) A:Hep G2(GTX27900) 7.5% SDS PAGE GTX101336 diluted at 1:1500
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REFERENCE
REVIEW
APP antibody [C2C3], C-term
Cat. No. GTX101336
Rating
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( Average 5 based on 2 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 10-Sep-2021
Application Tested :
IHC-Fr
Sample Species :
Rat
Sample :
hippocampus
Blocking :
1% BSA in PBST, RT, 1Hr
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
1% Triton and Citrate Buffer
Primary Antibody :
1:100, 4°C, > 12Hr
Date :
Beished Shabangu submitted on 30-Oct-2019
Application Tested :
WB
Sample Species :
Ms
Sample :
N2a cells
Blocking :
5% Milk, 25°C, 1Hr
Primary Antibody :
1:3000, 4°C, 1Hr