GeneTex
United States (US)

APP antibody [C2C3], C-term

GTX101336_39694_20160204_WB_19011506_478.jpg
GTX101336_39694_IHC_19011506_742.jpg
GTX101336_39694_20160325_IFA_19011506_935.jpg
GTX101336_39694_20160106_IHC-P_R_19011506_611.jpg
GTX101336_WB_19011506_557.jpg
GTX101336_WB_M_19011506_616.jpg

Cat No. GTX101336

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human APP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN2 antibody, APP antibody, alzheimer disease amyloid protein antibody, beta-amyloid peptide antibody, amyloid beta A4 protein antibody, peptidase nexin-II antibody, protease nexin-II antibody, cerebral vascular amyloid peptide antibody, preA4 antibody, amyloid beta (A4) precursor protein antibody, beta Amyloid antibody

Cellular Localization

Membrane , Membrane , clathrin-coated pit

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101336_39694_20160204_WB_19011506_478.jpg

GTX101336 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody [C2C3], C-term (GTX101336) diluted at 1:500.

GTX101336_39694_IHC_19011506_742.jpg

GTX101336 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP(GTX101336) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101336_39694_20160325_IFA_19011506_935.jpg

GTX101336 ICC/IF Image

APP antibody [C2C3], C-term detects APP protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APP protein stained by APP antibody [C2C3], C-term (GTX101336) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101336_39694_20160106_IHC-P_R_19011506_611.jpg

GTX101336 IHC-P Image

APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
APP antibody [C2C3], C-term (GTX101336) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101336_WB_19011506_557.jpg

GTX101336 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX101336 diluted at 1:1500

GTX101336_WB_M_19011506_616.jpg

GTX101336 WB Image

Sample (30 ug of whole cell lysate)
A:NIH-3T3 7.5% SDS PAGE
GTX101336 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169