Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF5 antibody (GTX104783) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ARF5 antibody detects ARF5 protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARF5 stained by ARF5 antibody (GTX104783) diluted at 1:500.
Blue: Hoechst 33342 staining.

ARF5 antibody detects ARF5 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ARF5 antibody (GTX104783) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min