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ARID2 antibody

Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX01135
Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX01135
Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX01135
Anti-ARID2 antibody used in Western Blot (WB). GTX01135

Cat No. GTX01135

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

197 kDa. ( Note )

Product Note

Superfamily members of ARID2 are not reactive to this antibody.

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence of human ARID2 (DERRKGLAFLDELRQFHHSR).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AT-rich interaction domain 2 , ARID2 , ATrich interaction domain 2 , BAF200 , p200

Cellular Localization

Nucleus

Background

This gene encodes a member of the AT-rich interactive domain (ARID)-containing family of DNA-binding proteins. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and chromatin structure modification. This protein functions as a subunit of the polybromo- and BRG1-associated factor or PBAF (SWI/SNF-B) chromatin remodeling complex which facilitates ligand-dependent transcriptional activation by nuclear receptors. Mutations in this gene are associated with hepatocellular carcinomas. A pseudogene of this gene is found on chromosome1. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX01135

GTX01135 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX01135 ARID2 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX01135

GTX01135 IHC-P Image

IHC-P analysis of rat testis tissue using GTX01135 ARID2 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX01135

GTX01135 IHC-P Image

IHC-P analysis of human sarcoma tissue using GTX01135 ARID2 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-ARID2 antibody used in Western Blot (WB). GTX01135

GTX01135 WB Image

WB analysis of various sample lysates using GTX01135 ARID2 antibody.
Lane 1 : THP-1 whole cell lysates
Lane 2 : K562 whole cell lysates
Lane 3 : Caco-2 whole cell lysates
Lane 4 : HL-60 whole cell lysates
Lane 5 : A549 whole cell lysates
Lane 6 : rat testicular issue lysates
Lane 7 : mouse testicular issue lysates
Dilution : 0.5μg/ml
Loading : 50μg of sample under reducing conditions

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379