Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114264 diluted at 1:1000

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ARL3 antibody [N1C3] (GTX114264) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.