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ASFV p54 antibody [HL1219]

Recombinant antibody
Anti-ASFV p54 antibody [HL1219] used in Western Blot (WB). GTX636563
Anti-ASFV p54 antibody [HL1219] used in ELISA (ELISA). GTX636563
Anti-ASFV p54 antibody [HL1219] used in ELISA (ELISA). GTX636563

Cat No. GTX636563

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1219

Isotype

IgG

Application

WB, ELISA, Sandwich ELISA

Reactivity

African swine fever virus
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636562, Detection : GTX636563.
Please notice that GTX636563 needs to be conjugated to HRP when paired with GTX636562. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910005

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-ASFV p54 antibody [HL1219] used in Western Blot (WB). GTX636563

GTX636563 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1219] (GTX636563) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASFV p54 antibody [HL1219] used in ELISA (ELISA). GTX636563

GTX636563 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1218] (GTX636562) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1219] (GTX636563) (0.1 μg/mL)
Please notice that GTX636563 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636562. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1219] used in ELISA (ELISA). GTX636563

GTX636563 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant recombinant HEK293 expressed, full-length ASFV p54 (40-0.63 nM). Coated protein was probed with ASFV p54 antibody [HL1219] (GTX636563) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

There are currently no references for ASFV p54 antibody [HL1219] (GTX636563). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations