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ASIC3 antibody

Anti-ASIC3 antibody used in Flow cytometry (FACS). GTX01177
Anti-ASIC3 antibody used in Western Blot (WB). GTX01177

Cat. No. GTX01177

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($439)
See all ASIC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
FACS 1-3μg/1x10⁶ cells
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Product Note

Superfamily members of ASIC3 are not reactive to this antibody.

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg Thimerosal, 0.05mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3(56-73aa FLYQVAERVRYYREFHHQ).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acid sensing ion channel subunit 3 , ACCN3 , ASIC3 , DRASIC , SLNAC1 , TNaC1

Cellular Localization

Cell membrane,Cytoplasm

Background

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 2 has been observed as proton-gated channels sensitive to gadolinium. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-ASIC3 antibody used in Flow cytometry (FACS). GTX01177

GTX01177 FACS Image

FACS analysis of U937 cells using GTX01177 ASIC3 antibody.
Blue : Primary antibody
Green : Isotype control
Red : No antibody control
Antibody amount : 1μg/1x10⁶ cells

Anti-ASIC3 antibody used in Western Blot (WB). GTX01177

GTX01177 WB Image

WB analysis of various sample lysates using GTX01177 ASIC3 antibody.
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Rat Testis Tissue Lysate
Lane 3 : U87 Cell Lysate
Lane 4 : NEURO Cell Lysate
Lane 5 : SMMC Cell Lysate
Loading : 50μg of sample under reducing conditions
Dilution : 0.5 μg/mL

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 439