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ASIC3 antibody

Anti-ASIC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10354

Cat No. GTX10354

Host Guinea pig
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-Fr
Reactivity Rat
Package
50 μl ($319)
APPLICATION

Application Note

ICC: Use at a dilution of 1/100. IHC-Fr: Use at a dilution of 1/100. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

59 kDa. ( Note )

Positive Control

ASIC3 transfected cells and primary neuronal cells
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.05% Sodium Azide; Constituents: 1% BSA, PBS. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Synthetic peptide: TASLDPDDFDPEPSDPLGSP, corresponding to amino acids 285-304 of extracellular domain of Rat ASIC3.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acid Sensing Ion Channel Subunit 3 , Accn3 , Asic3

Cellular Localization

Plasma membrane

Background

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, containing many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and is thought to play an important role in detecting lasting pH changes. In addition, a heteromeric association between this member and ACCN1 has been observed as proton-gated channels sensitive to gadolinium. Three transcript variants are generated following alternative splicing of this gene each encoding distinct isoforms.

Database

Research Area

DATA IMAGES
Anti-ASIC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10354

GTX10354 ICC/IF Image

ASIC3 expression compared to other nociceptive ion channels. DRG sections double-labeled for ASIC3 (red) and either P2X3 (green, A) orTRPV1 (green, B); double-labeled cells are yellow or orange. Circle charts show the relative distribution of the three receptors among all DRG neurons. ASIC3 and P2X3 expression overlapped in only 4% of neurons, while some 35% of ASIC3-positive neurons also express TRPV1. The rare cells positive for both ASIC3 and P2X3 were large (eg. cell at lower left in A). 1406 cells counted for A; 995 for B. C, D, electrophysiological recordings reveal a qualitatively similar expression pattern for currents conforming to ASIC3, P2X3, or TRPV1. The cell that generated the currents in C counts as a co-expressor of ASIC3 and P2X3, fitting into the yellow bin in the circle plot. The cell in D is a co-expressor of ASIC3 and TRPV1. Cells were counted positive if they had at least 0.3 nA of current in response to pH 6.8 (ASIC3+), 50 μM ATP (P2X3+; transient current only), or 1 μM capsaicin (TRPV1). 182 cells recorded for C; 169 for D.

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Package List Price ($)
$ 319