Phosphate buffered saline, pH 7.3, 50% glycerol and 150mM NaCl containing 0.02% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
The antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 83 (G-S-S^P-V-G).
Affinity purified with antigen
From polyclonal serum
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Mitogen-Activated Protein Kinase Kinase Kinase 5,Ask1,Mapkkk5,Mekk5,Map3K5
Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF-a induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.