Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ASXL1 antibody (GTX127284) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ASXL1 antibody detects ASXL1 protein at nucleus in human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma.
ASXL1 antibody (GTX127284) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ASXL1 antibody detects ASXL1 protein by western blot analysis.
A. 50 μg 293T whole cell lysate/extract
B. 50 μg whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells
ASXL1 antibody (GTX127284) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.