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ATG4A antibody

Anti-ATG4A antibody used in IHC (Paraffin sections) (IHC-P). GTX87155
Anti-ATG4A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87155

Cat. No. GTX87155

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all ATG4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human ATG4A (81-130).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

APG4A , ATG4A , AUTL2 , autophagy related 4A cysteine peptidase , autophagy related 4A, cysteine peptidase

Cellular Localization

Cytoplasm

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ATG4A antibody used in IHC (Paraffin sections) (IHC-P). GTX87155

GTX87155 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX87155 ATG4A antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ATG4A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87155

GTX87155 ICC/IF Image

ICC/IF analysis of A549 cells using GTX87155 ATG4A antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399