GeneTex
  • Country / Location Selection

United States (US)

ATM antibody [2C1]

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103
Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103
Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103
Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103
Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103
Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103
Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

Cat No. GTX70103

Host

Mouse

Clonality

Monoclonal

Clone Name

2C1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, IP, ELISA, ChIP assay, IHC

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($329)
PRODUCT

Summary

ATM antibody [2C1] is a mouse monoclonal antibody developed by Dr. Eva Lee’s lab at the University of Texas Health Science Center at San Antonio (PMID: 8969240). It is a well-validated and highly cited reagent to detect ATM protein, which is a nuclear serine/threonine kinase that plays a pivotal role in DNA damage sensing and repair.

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa.
For IHC-P: Use at 5 μg/mL. Antigen retrieval in Citrate buffer is recommended.
For IP: Use at a concentration of 1-10 μg/ml.
For ICC/IF: Please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For FACS:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

351 kDa. ( Note )

Positive Control

SK-N-SH , HeLa , HeLa nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

Purification

Purified by antigen-affinity chromatography.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_368161

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

GTX70103 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103

GTX70103 IHC-P Image

Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

GTX70103 WB Image

Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody (GTX70103) by western blot or immunoprecipitation.

Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103

GTX70103 IHC-P Image

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ATM antibody [2C1] used in IHC (Paraffin sections) (IHC-P). GTX70103

GTX70103 IHC-P Image

ATM antibody [2C1] detects ATM protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ATM stained by ATM antibody [2C1] (GTX70103) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

GTX70103 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:1000.

Anti-ATM antibody [2C1] used in Western Blot (WB). GTX70103

GTX70103 WB Image

HeLa whole cell extract and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] (GTX70103) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ATM antibody [2C1] Cat No. GTX70103

Rating ( Average 5 based on 12 users reviews)
Application
Western Blot(WB) ( Average 5 based on 11 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 24086335
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 24086335
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 27074761
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2010.PMID: 20356374
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 24933654
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 24086335
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu, Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 23667485
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25826455
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Res in 2018.PMID: 29844578
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25826455
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Aging Cell in 2015.PMID: 25645366
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Res in 2018.PMID: 29844578
Application Reference
SDS
PBS.pdf