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ATP5A1 antibody [C2C3], C-term

Overexpression
GTX101741_40128_IHC_M_2_19011506_212.jpg
GTX101741_40128_IHC_M_19011506_493.jpg
GTX101741_41871_20160808_IHC-Fr_19011506_342.jpg
GTX101741_41871_20160818_WB_M_R_19011506_105.jpg
GTX101741_41871_20141024_WB_19011506_918.jpg
GTX101741_41871_20170524_IFA_19011506_407.jpg
GTX101741_41871_20180831_WB_B_19011506_585.jpg
GTX101741_41871_20170511_WB_19011506_550.jpg
GTX101741_40128_IHC_19011506_121.jpg

Cat No. GTX101741

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , K562 , THP-1 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATP5A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atp Synthase F1 Subunit Alpha,Atp5A,Atp5A1,Atp5Al2,Atpm,Coxpd22,Hel-S-123M,Mc5Dn4,Mom2,Omr,Orm,Hatp1,Atp5F1A

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101741_40128_IHC_M_2_19011506_212.jpg

GTX101741 IHC-P Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at membrane on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101741_40128_IHC_M_19011506_493.jpg

GTX101741 IHC-P Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101741_41871_20160808_IHC-Fr_19011506_342.jpg

GTX101741 IHC-Fr Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101741_41871_20160818_WB_M_R_19011506_105.jpg

GTX101741 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:1000.

GTX101741_41871_20141024_WB_19011506_918.jpg

GTX101741 WB Image

ATP5A1 antibody detects ATP5A1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody (GTX101741) diluted by 1:2000.

GTX101741_41871_20170524_IFA_19011506_407.jpg

GTX101741 ICC/IF Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101741_41871_20180831_WB_B_19011506_585.jpg

GTX101741 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101741_41871_20170511_WB_19011506_550.jpg

GTX101741 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody (GTX101741) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101741_40128_IHC_19011506_121.jpg

GTX101741 IHC-P Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169