Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP7A antibody [GT128] (GTX642327) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)