GeneTex
United States (US)

AUF1 antibody [N1C1]

GTX101813_40660_WB_19011506_233.jpg
GTX101813_40660_20150410_IFA_19011506_303.jpg
GTX101813_40660_20141205_IFA_19011506_444.jpg

Cat No. GTX101813

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AUF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heterogeneous Nuclear Ribonucleoprotein D,Auf1,Auf1A,Hnrpd,P37,Hnrnpd0,Hnrnpd

Cellular Localization

Nucleus

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq]

Database

DATA IMAGES
GTX101813_40660_WB_19011506_233.jpg

GTX101813 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX101813 diluted at 1:1000

GTX101813_40660_20150410_IFA_19011506_303.jpg

GTX101813 ICC/IF Image

AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AUF1 protein stained by AUF1 antibody [N1C1] (GTX101813) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX101813_40660_20141205_IFA_19011506_444.jpg

GTX101813 ICC/IF Image

AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AUF1 protein stained by AUF1 antibody [N1C1] (GTX101813) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
REVIEW

AUF1 antibody [N1C1] Cat No. GTX101813

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Panc1 cells
Amount used : 30μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169